ABSTRACT
Objective To investigate the significance of chromoendoscopy with acetic acid and indigo carmine dye in the diagnosis of early gastric neoplasia and precancerous lesion.Methods After conventional electronic endoscopic diagnosis,From 2011 fanuay to 300 patients of suspicious lesions were randomly divided into endoscopic dye group and control group,each was 150 cases.Patients of endoscopic dye group were directly performed biopsy after acetic acid and indigo carmine dye,while in control group were performed biopsy after only indigo carmine dye.Results In the endoscopic dye group,16 early neoplasia (10.7％),15 severe atypical hyperplasia (10.0％),79 moderate atypical hyperplasia or Intestinal metaplasia (52.7％) and 40 chronic gastritis were found.In the control group,5 early neoplasia (3.3％),10 severe atypical hyperplasia (6.7％),42 moderate atypical hyperplasia or Intestinal metaplasia(28.0％) and 93 chronic gastritis were found.Early gastric cancer and precancerous lesion detection rate of endoscopic dye was significantly higher than that of the control group,and the difference was statistically significant (P ＜ 0.001).After samples were stained with acetic acid and indigo carmine dye,the image of the mucosa of early cancer and severe atypical hyperplasia were faded mucosa,and the image of moderate atypical hyperplasia and Intestinal metaplasia was dyed asymmetrical.Chronic gastritis and normal mucosa was showed dyed equality.Conclusion Chromoendoscopy with acetic acid and indigo carmine dye improve the detection rate of early gastric cancer and precancerous lesion,thereby of high application value.
ABSTRACT
Objective To explore the association of expression of transporter associated with antigen processing(TAP1),tumor necrosis factor-alpha(TNF-α)with occurrence and development of HBV-related hepatocellular carcinoma(HCC).Methods The expression of TAP1 and TNF-α in 35 liver carcinoma tissues,and 10 normal liver tissues were detected with immunohistochemical EnVision method,The expression and clinical significance of TAP1,TNF-α in HBV-related HCC were analyzed.Furthermore,the association of respective expression quantity with pathological typing of HCC was analyzed.Results The expression of TAP1,TNF-α was negative in hepatocytes of normal liver tissues.91.4％ of HCC tissues expressed TAP1.There was significantly statistical difference in expression of TAP1 between HCC tissues and the normal hepatic tissues(P ＜ 0.05);60％ of HCC tissues expressed TNF-α.There was statistical difference in expression of TNF-α between HCC and the normal hepatic tissues(P ＜ 0.05),and the positive expression rate of TNF-α in well-differenciated HCC tissues was significantly higher than that in poorly-differenciated ones(P ＜ 0.05).Conclusion TAP1-related MHC-Ⅰ restricted antigen processing pathway was possibly normal in HBV-related HCC.The expression quantity of TNF-α was associated with the occurrence and degree of pathological differentiation of HBV-related HCC.
ABSTRACT
The clinical significance of a myeloperoxidase (MPO) gene polymorphism and inducible nitric oxide synthase (iNOS) expression in cirrhotic patients with hepatopulmonary syndrome (HPS) was explored. Enrolled subjects were divided into three groups according to their disease/health conditions: the HPS group (cirrhotic patients with HPS; n=63), the non-HPS group (cirrhotic patients without HPS; n=182), and the control group (healthy subjects without liver disease; n=35). The distribution of the MPO -463 G/A genotype and its relationship with iNOS expression in a typical cell block from ascitic fluid were detected by immunohistochemistry and polymerase chain reaction-restricted fragment length polymorphism analysis (PCR-RFLP). In the HPS group, the partial pressure of oxygen in blood and ascitic fluid was significantly decreased (8.95+/-1.58 kPa and 6.81+/-0.95 kPa, respectively; both P<0.01), while the partial pressure of carbon dioxide significantly increased (4.62+/-0.20 kPa and 5.92+/-0.45 kPa, respectively; P<0.01). MPO and iNOS levels were significantly increased in the HPS group as compared with the non-HPS group. These increases were even more remarkable in ascitic fluid (41.36+/-11.62 and 13.23+/-4.81 mug/L; 10.27+/- 3.20 and 4.95+/-1.12 mug/L) than in blood (16.66+/-5.24 and 4.87+/-1.73 mug/L; 5.79+/-2.31 and 2.35+/-0.84 mug/L). The distribution of the MPO genotypes GG, GA, and AA were 76.2%, 22.2% and 1.6% in the HPS group, and 57.7%, 37.9% and 4.4% in the non-HPS group (P<0.05). The expression of iNOS was significantly higher in patients with the G alleles (G/G and G/A) (61.54%, 48/78) than in patients with A alleles (G/A and A/A) (38.46%, 30/78) (P<0.01). It was suggested that the expression levels of iNOS and MPO were correlated with HPS-induced hypoxemia. The MPO-463 G/A mutation might be a protective factor that prevents the development of HPS. The MPO might be involved in the regulation of iNOS expression. In humans, MPO pathways, the iNOS/NO system, and their interaction might have an impact on the occurrence and development of HPS.